Stepwise solid phase synthesis of uridylylated viral genome-linked peptides using uridylylated amino acid building blocks

Publication Type:

Journal Article

Authors:

Kriek, N.M.A.J.; Filippov, D.V.; van den Elst, H.; Meeuwenoord, N.J.; Tesser, G. I; van Boom, J.H.; van der Marel, G.A.

Source:

Tetrahedron, Volume 59, Number 9, p.1589-1597 (2003)

ISBN:

0040-4020

Keywords:

genome-linked peptides; uridylylated amino acid building blocks; solid phase peptide synthesis; rink amide resin; adenovirus-2 nucleoprotein; nucleopeptide fragment; poliovirus genome; nucleic-acids; rna-synthesis; protein; protection; strategy; gene

Abstract:

The uridylylated amino acid building blocks 2-cyanoethyl-(N-alpha-9-fluorenylmethoxy-carbonyl-tyrosin-4-yl)-(2',3'-di-O-acetyl-uridin-5'-yl) phosphate and 2-chlorophenyl-(N-alpha-fluorenyl-methoxycarbonyl-serin-3-yl)-(2',3'-di-O-acetyluridin-5'-yl) phosphate have been used successfully in an on-line SPPS of the VPgpU from the polio, coxsackie and cowpea mosaic virus. (C) 2003 Elsevier Science Ltd. All rights reserved.