Increased paramagnetic effect of a lanthanide protein probe by two-point attachment

Publication Type:

Journal Article

Source:

Journal of the American Chemical Society, Volume 129, Number 30, pp. 9292-+ (2007)

ISBN:

0002-7863

DOI Name (links to online publication)

10.1021/Ja0725201

Keywords:

residual dipolar couplings; pseudocontact shifts; nmr structures; macromolecules; complexes; dynamics; chelate; dota; ion

Abstract:

A lanthanide ligand is reported, amenable to be immobilized on a protein surface via cysteine residues. This probe yields large single shifts of the protein nuclei resonances in HSQC NMR spectra and causes significant alignment of the protein in the magnetic field, providing additional structural restraints. Anchoring the probe by two cysteine residues instead of one is highly beneficial for the size of its effects.

27/01/2009